Journal: Journal of Thoracic Disease
Article Title: Hepatocyte growth factor alleviates alcoholic cardiomyopathy through the Nrf2 pathway—a focus on iron metabolism
doi: 10.21037/jtd-2025-1111
Figure Lengend Snippet: Effects of exogenous HGF on ferroptosis in cardiomyocytes. (A) The effect of HGF on cell viability (CCK8); (B) the effect of HGF on GPX4; (C) the effect of HGF on LIP; (D) the effect of HGF on cell survival by calcein staining; (E) MDA concentrations of different HGF doses; (F) GPX4 concentrations of different HGF doses; (G) LIP levels of different HGF doses; (H) MDA concentrations following Nfe2l2 knockdown in ethanol-treated cells; (I) GPX4 concentrations following Nfe2l2 knockdown in ethanol-treated cells; (J) LIP levels following Nfe2l2 knockdown in ethanol-treated cells; (K) Cell survivals by calcein staining following Nfe2l2 knockdown in ethanol-treated cells. **, P<0.01; ns, P>0.05. CCK8, Cell Counting Kit-8; GPX4, glutathione peroxidase 4; HGF, hepatocyte growth factor; LIP, labile iron pool; MDA, malondialdehyde; MFI, mean fluorescence intensity; PBS, phosphate buffered saline; scramble, randomly interrupted sequence; siRNA, small interfering RNA.
Article Snippet: The Bicinchoninic Acid protein assay kit (71285-M), calcein-AM (206700) and 3-hydroxy-1,2-dimethyl-4(1H)-pyridone (379409) from Sigma-Aldrich (St. Louis, USA); the Trizol reagent, RIPA protein lysis buffer, malondialdehyde (MDA) kit (S0131S), Cell Counting Kit-8 (CCK8), calcein staining kit (C2013S) and hematoxylin-eosin (HE) staining kit (C0105S) from Beyotime (Shanghai, China); the glutathione peroxidase 4 (GPX4) (CSB-EL009869RA) and HGF (CSB-E07346r) enzyme-linked immunosorbent assay (ELISA) kit from Cusabio (Wuhan, China).
Techniques: Staining, Knockdown, Cell Counting, Fluorescence, Saline, Sequencing, Small Interfering RNA